human t cell nucleofector kit (Amaxa)

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Amaxa human t cell nucleofector kit
Human T Cell Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+t+cell+nucleofector+kit/electroporation/us12421294-167-8-13
Average 86 stars, based on 1 article reviews

human t cell nucleofector kit - by Bioz Stars, 2026-09

86/100 stars

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Transfection:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. Alternatively, for RNAi-mediated TFEB silencing CTLs were transiently transfected at day 2 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human TFEB-specific (#FE5L009798000010) or negative control (#FE5D0018101020) siRNAs (Dharmacon, Lafayette, Colorado, USA) (150 ng × 106 cells). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: A gRNA transcription template was prepared by PCR amplification using the PX458 construct as a template and the primers listed in Table , and then transcribed in vitro using the HiScribe T7 high yield RNA synthesis kit (NEB, Ipswich, Massachusetts, USA). gRNAs were purified by RNA clean & concentration (Zymo Research, Irvine, California, USA). .. Freshly isolated CD8 + T cells were transfected using the Human T cell nucleofector kit and the program V-024 of the Nucleofector II system (Amaxa Biosystems, Euroclone, Milan, Italy) for unstimulated T cells, with ribonucleoprotein complexes formed mixing 10 μg of Alt-R Cas9 Nuclease V3 protein (IDT, Coralville, Iowa, USA) and 6 μg of gRNA. .. Cells were then activated with anti-CD3/CD28 magnetic beads in AIM V culture medium (ThermoFisher Scientific) supplemented with 10% BCS and 500 U/ml of human IL-2 for 48 h. After removal of the beads, cells were expanded in AIM V 10% BCS with 50 U/ml human IL-2.

Article Title: Truncated epiderimal growth factor receptor (EGFRt) for transduced t cell selection
Article Snippet: .. PBMC were resuspended in nucleofection solution using the Human T cell Nucleofector kit (Amaxa Inc., Gaithersberg, MD), and 5×107 cells were aliquoted into 0.2-cm cuvettes containing 10 μg HygroR-pp65_pEK (or pmaxGFP from Amaxa Inc., as a transfection control) in a final volume of 100 μL/cuvette, and electroporated using the Amaxa Nucleofector I (Amaxa Inc.), program U-14, after which cells were allowed to recover for 6 hours at 37° C. prior to γ-irradiation (1200 cGy). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. To modulate TFEB expression or function CTLs at day 5 of differentiation were transiently transfected using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Bioystems) for activated T cells, with 1 μg × 106 cells of pEGFP-N1 or pEGFP-N1-TFEB plasmids. ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. Alternatively, for RNAi-mediated TFEB silencing CTLs were transiently transfected at day 2 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human TFEB-specific (#FE5L009798000010) or negative control (#FE5D0018101020) siRNAs (Dharmacon, Lafayette, Colorado, USA) (150 ng × 10 6 cells). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: A gRNA transcription template was prepared by PCR amplification using the PX458 construct as a template and the primers listed in Table S2, and then transcribed in vitro using the HiScribe T7 high yield RNA synthesis kit (NEB, Ipswich, Massachusetts, USA). gRNAs were purified by RNA clean & concentration (Zymo Research, Irvine, California, USA). .. Freshly isolated CD8+ T cells were transfected using the Human T cell nucleofector kit and the program V-024 of the Nucleofector II system (Amaxa Biosystems, Euroclone, Milan, Italy) for unstimulated T cells, with ribonucleoprotein complexes formed mixing 10 μg of Alt-R Cas9 Nuclease V3 protein (IDT, Coralville, Iowa, USA) and 6 μg of gRNA. .. Cells were then activated with anti-CD3/CD28 magnetic beads in AIM V culture medium (ThermoFisher Scientific) supplemented with 10% BCS and 500 U/ml of human IL-2 for 48 h. After removal of the beads, cells were expanded in AIM V 10% BCS with 50 U/ml human IL-2.

Negative Control:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. Alternatively, for RNAi-mediated TFEB silencing CTLs were transiently transfected at day 2 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human TFEB-specific (#FE5L009798000010) or negative control (#FE5D0018101020) siRNAs (Dharmacon, Lafayette, Colorado, USA) (150 ng × 106 cells). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. Alternatively, for RNAi-mediated TFEB silencing CTLs were transiently transfected at day 2 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human TFEB-specific (#FE5L009798000010) or negative control (#FE5D0018101020) siRNAs (Dharmacon, Lafayette, Colorado, USA) (150 ng × 10 6 cells). ..

esiRNA:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Control:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Luciferase:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: .. For RNAi-mediated IFT20 silencing, CTLs were transiently transfected at day 6 of differentiation using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems) for activated cells with human IFT20-specific endoribonuclease-prepared siRNAs (esiRNA)(Sigma-Aldrich) and unrelated control Renilla luciferase (RLUC) esiRNA (Sigma-Aldrich). ..

Isolation:

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B
Article Snippet: A gRNA transcription template was prepared by PCR amplification using the PX458 construct as a template and the primers listed in Table , and then transcribed in vitro using the HiScribe T7 high yield RNA synthesis kit (NEB, Ipswich, Massachusetts, USA). gRNAs were purified by RNA clean & concentration (Zymo Research, Irvine, California, USA). .. Freshly isolated CD8 + T cells were transfected using the Human T cell nucleofector kit and the program V-024 of the Nucleofector II system (Amaxa Biosystems, Euroclone, Milan, Italy) for unstimulated T cells, with ribonucleoprotein complexes formed mixing 10 μg of Alt-R Cas9 Nuclease V3 protein (IDT, Coralville, Iowa, USA) and 6 μg of gRNA. .. Cells were then activated with anti-CD3/CD28 magnetic beads in AIM V culture medium (ThermoFisher Scientific) supplemented with 10% BCS and 500 U/ml of human IL-2 for 48 h. After removal of the beads, cells were expanded in AIM V 10% BCS with 50 U/ml human IL-2.

Article Title: IFT20 regulates TFEB-dependent lytic granule biogenesis in cytotoxic T lymphocytes by orchestrating the MPR-dependent transport of granzyme B.
Article Snippet: A gRNA transcription template was prepared by PCR amplification using the PX458 construct as a template and the primers listed in Table S2, and then transcribed in vitro using the HiScribe T7 high yield RNA synthesis kit (NEB, Ipswich, Massachusetts, USA). gRNAs were purified by RNA clean & concentration (Zymo Research, Irvine, California, USA). .. Freshly isolated CD8+ T cells were transfected using the Human T cell nucleofector kit and the program V-024 of the Nucleofector II system (Amaxa Biosystems, Euroclone, Milan, Italy) for unstimulated T cells, with ribonucleoprotein complexes formed mixing 10 μg of Alt-R Cas9 Nuclease V3 protein (IDT, Coralville, Iowa, USA) and 6 μg of gRNA. .. Cells were then activated with anti-CD3/CD28 magnetic beads in AIM V culture medium (ThermoFisher Scientific) supplemented with 10% BCS and 500 U/ml of human IL-2 for 48 h. After removal of the beads, cells were expanded in AIM V 10% BCS with 50 U/ml human IL-2.

Expressing: